@article {1886,
	title = {Development of anin situ assay to detect bacterial pathogens of the red algaGracilaria gracilis (Stackhouse) Steentoft, Irvine et Farnham},
	journal = {Journal of Applied PhycologyJournal of Applied Phycology},
	volume = {8},
	number = {4},
	year = {1996},
	month = {1996/07/01},
	pages = {409-414},
	abstract = {Since current protocols were found to be inadequate for the identification of bacteria pathogenic toGracilaria gracilis, an assay was developed which made use of {\textquoteleft}axenic{\textquoteright} algae in order to attribute disease symptoms directly to the presence of a specific bacterial isolate. However, this assay proved to be as unreliable as existing procedures and failed to satisfy Koch{\textquoteright}s postulates. A second pathogenicity assay was developed which proved more reliable in that each bacterial strain consistently induced a particular symptom in the infected algal thallus. The bacterial strain LS2i was identified as a possible pathogen ofG. gracilis using this assay. However, this assay did not satisfy Koch{\textquoteright}s criteria for establishing an unequivocal link between the observed disease symptoms and strain LS2i, since significant bacterial contamination of the test alga occurred. A third protocol generated consistent results and satisfied Koch{\textquoteright}s postulates, enabling the identification of six pathogens ofG. gracilis. All the pathogenic bacterial strains were agarolytic.},
	isbn = {1573-5176},
	author = {Jaffray, Ann E. and Coyne, Vernon E.}
}
